By M. Cristina Vega
This e-book offers complicated expression applied sciences for the creation of protein complexes. considering that complexes lie on the center of recent biology, the expression, purification, and characterization of huge quantities of fine quality protein complexes is essential for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and bug cells to advanced reconstitution from person subunits, this publication deals precious insights and assistance for winning protein expressionists.
Across numerous sections readers will realize present possibilities for the construction of protein complexes in bacterial platforms (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, crops and algae. advanced reconstitution from separately purified subunits or subcomplexes is mentioned as a complementary technique. a final part introduces in short many of the biophysical and structural characterization concepts for macromolecular complexes utilizing cutting-edge resolution scattering and nuclear magnetic resonance.
This paintings is a guided travel over probably the most robust and winning protein expression applied sciences, with a spotlight on co-expression and high-throughput purposes. it really is addressed to every person drawn to the creation and characterization of macromolecular complexes, from college scholars who wish an obtainable description of the main co-expression structures to researchers in biomedicine and the lifestyles sciences looking for an updated survey of obtainable technologies.
Read Online or Download Advanced Technologies for Protein Complex Production and Characterization (Advances in Experimental Medicine and Biology) PDF
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Extra info for Advanced Technologies for Protein Complex Production and Characterization (Advances in Experimental Medicine and Biology)
Advanced Technologies for Protein Complex Production and Characterization (Advances in Experimental Medicine and Biology) by M. Cristina Vega
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